NAD+

Nicotinamide adenine dinucleotide for cellular and mitochondrial research. For in-vitro research only.

• Crystal structures of a human flavoprotein show the nicotinamide moiety held away from the flavin when the oxidized cofactor is bound and stacked directly on the isoalloxazine ring when the reduced form is bound, so the two oxidation states occupy the site differently. [1]
• A bacterial Nudix hydrolase resolved with the intact cofactor and with its cleavage product strongly prefers the RNA-linked form of the cofactor over the free dinucleotide, which separates the free molecule from the RNA cap species. [2]
• A genetically encoded fluorescent biosensor put free cofactor concentrations in nucleus, cytoplasm and mitochondria close to the Michaelis constants of the sirtuins and ADP-ribosyltransferases resident in each compartment. [3]
• In cells engineered to overconsume the cofactor in one compartment at a time, the lowered concentration kinetically restricted consumption while the biosynthesis rate stayed unchanged, and mitochondria buffered the shortfall by import through SLC25A51. [4]

Compound

NAD+

Purity

99.30% (HPLC)

Identity

Confirmed by MS

Appearance

White lyophilate

Presentation

500 mg · 1000 mg

Catalog / lot

RV-0004 / RV-24-0004-2

For research use only. Not for human or veterinary use. Not a drug, food, cosmetic, or supplement.

Review the laboratory, lot identifier, and reported results in this document. Keep the lot identifier with your research records.